Coding

Part:BBa_K3793011:Design

Designed by: Rupesh Balaji Jayachandran, Enrique de Dios Mateos   Group: iGEM21_Aalto-Helsinki   (2021-10-21)


GST tag with enterokinase cleavage site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 100


Design Notes

The GST tag can be used for both N- and C-Terminal tagging. Enterokinase site can be added accordingly, for our design we had opt for the C-Terminal tagging of our target protein since we already had a 6x HIS tag in the N-Terminal.


Source

The sequence was referenced from genome of Schistosoma japonicum (GenBank accession: M14654)

References